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Showing 1,861–1,872 of 1,994 articles

PubMed

The Infestation of Supplied Mincemeat to Sarcocystis Species and Their Polymorphism Using PCR-SSCP Method.

Background: Sarcocystis spp. is zoonotic parasitic pathogen endangering the safety of meat and derived meat products. This study was carried on for determination of presence of Sarcocystis species in mincemeat in Tabriz (Iran) using single-stranded conformation polymorphism (SSCP) method. Methods: Thirty samples of mixed ground meat were collected from butchers and DNA was extracted. The 18S rRNA gene fragment of S. cruzi and S. hominis species in the extracted DNA amplified by PCR using one...

Pcr
Nematollahi A, Firuz Amandi M, Emdadi M•
PubMed

Role of PDGF-BB in Oral Squamous Cell Carcinoma through PI3/AKT/mTOR Pathway: An Integrated Computational and Real-Time PCR-Based Approach.

Objective: This study investigates the role of PDGF-BB in oral squamous cell carcinoma (OSCC) and its impact on the PI3K/AKT/mTOR pathway. The goal is to elucidate the expression levels analysis of PDGF-BB and signaling molecules in OSCC pathogenesis, potentially identifying novel biomarkers or therapeutic targets. Methods: A combined approach involving in silico and experimental methods was employed. Protein sequence data for PDGF-BB were obtained from UniProt, and protein-protein interactio...

Pcr
Benitha J G, Ramani P, Jayaraman S, Ramasubramaniam A, Sagar S•
PubMed

Correction to "Genetic Diversity and Ecological Distribution of Bulinus Populations in Southern Benin Using PCR-RFLP Analysis".

E. Alabi, A. C. Do, J. Nougbode, et al., “Genetic Diversity and Ecological Distribution of Bulinus Populations in Southern Benin Using PCR‐RFLP Analysis,” BioMed Research International no. 1 (2026): 5536391, https://doi.org/10.1155/bmri/5536391. In the Methodology Section, Chapter 2.4, Statistical Analyses, References [28, 29] were included in error. The authors did not present the formulas used for the calculation of the genetic diversity parameters. The correct references are [42, 43]. We ap...

Pcr
PubMed

Rapid Identification and Differential Detection of Infectious Bursal Disease Virus by an Innovative ARMS-qRT-PCR Assay.

Infectious bursal disease (IBD), caused by IBD virus (IBDV), is a major threat to the poultry industry, with the cocirculation of multiple genotyping strains complicating the diagnosis and control. Since the differences of antigenicity among different genotyping and their differences with the commonly used vaccine strains, rapid and accurate identification and differential detection are crucial for surveillance and control of IBDV. In this study, a novel amplification refractory mutation system...

Pcr
Wang W, Qiao Y, Wu J, Kong R, Zhang Y, Shi J, Chen J, Wang Z, Zhao H, Wei T, Huang T, Huang J, Mo M, He X, Wei P•
PubMed

Development and Optimization of a PCR-RFLP Method for Differentiation of Aspergillus flavus From Aspergillus parasiticus.

Introduction: Aflatoxins are highly toxic secondary metabolites produced by several Aspergillus species, pose a global threat to food and feed safety, making rapid and reliable identification of aflatoxigenic species essential for effective surveillance and risk mitigation. Here, we report the development and validation a simple polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) assay that discriminates between the closely related species Aspergillus flavus and Asperg...

Pcr
Sohrabi N, Taghizadeh M•
PubMed

What is next for Hematology, Hemotherapy and Cell Therapy? A message and an invitation from incoming editors.

Along with the start of 2026, we assume the great responsibility of continuing the work of editors who in the past brought our journal Hematology, Hemotherapy and Cell Therapy (HTCT) to the place it occupies today in the scientific publishing landscape. Throughout this time, the journal has achieved national and international recognition, illustrated, but not exclusively defined, by indexing and impact metrics. It is fair to say today that, nationally, HTCT has ceased to be a channel for publish...

Hematology
De Paula EV, Dinardo CL•
PubMed

Application of Pain Management After Lumbar Spine Surgery: A Bibliometric Analysis of Randomized Controlled Trials.

Study Design Bibliometric analysis. Objective To optimize pain management and identify future research trends through bibliometric analysis. Summary of Background Data Postoperative lumbar spine pain is multifactorial and often persists despite successful surgery, reducing patients’ quality of life, increasing healthcare use, and increasing the risks of opioid dependence and psychological distress. Methods Data from eligible studies were extracted through a comprehensive search and variou...

Pain Management
Li D, Gan Y, Li X, Han S, Zhu H, Yang C, Zhang Y, Huang R, Li Q, Tudi A, Meng B, Liu C, Yu X•
PubMed

Pharmacological Therapies for Pain Management in Patients With Amputation: A Systematic Review and Network Meta-Analysis.

Objective To evaluate the efficacy and safety of pharmacological therapies for pain management in postamputation patients through a systematic review and network meta‐analysis of existing evidence. Methods This systematic review and network meta‐analysis (NMA) was conducted following the PRISMA‐NMA guidelines and registered in PROSPERO (CRD42024500600). In September 2024, two independent reviewers searched the Cochrane Library, LILACS, PubMed/MEDLINE, SciELO, ScienceDirect, Web of Science, Sc...

Pain Management
Leal NTB, Marinho LRDF, Pereira VDSL, Pontes BCR, Sarmento ACA, Medeiros KS, Ribeiro KRB, Dantas RAN, Dantas DV•
PubMed

Understanding the Evidence-to-Practice Gap in Persistent Pain Management.

Purpose Despite advances in pain science education, evidence‐based approaches to chronic pain are not consistently translated into physical therapy practice. Physical therapist (PT) clinical instructors (CIs) occupy a unique role as both clinicians and mentors of future practitioners. This study examined PT CIs’ pain knowledge, pain‐related beliefs, confidence, and self‐reported use of pain neuroscience education (PNE) and explored perceived barriers to implementing evidence‐informed persistent...

Pain Management
Henderson VK, Covert C, Pontiff R, Greenwood R•
PubMed

Artificial Intelligence and Connected Technologies in Chronic Pain Management: A Scoping Review.

Background Chronic pain affects over 1.5 billion people worldwide, facing persistent challenges in assessment subjectivity, treatment variability, and scalability (i.e., the limited ability to deliver individualized care to large populations within current clinical systems). Artificial intelligence (AI) and connected health technologies have transitioned from experimental tools to clinical reality in pain management. Objective This scoping review maps current AI applications, identifies imple...

Pain Management
Elhomsy P, Reliquet B, Sudria F•
PubMed

Nurse Competencies, Patient Characteristics, and Pain Management Protocol Adherence in Intensive Care Units: A Multicenter Cross-Sectional Study Accounting for ICU Clustering.

Aim(s) To examine the associations between nurse competencies (knowledge, self‐efficacy, and attitude), patient characteristics, and pain management protocol adherence in adult intensive care units, while accounting for between‐ICU clustering. Background Adherence to pain management protocols remains suboptimal despite available guidelines. Nurse competencies are essential for evidence‐based practice, yet their translation into clinical performance may depend on contextual factors. ICU‐level...

Pain Management
Shafie Motlagh Kashani Y, Shali M, Esmaieli M, Haghani S•
PubMed

Defining Wound Care Learning Objectives for Medical Students: A Multidisciplinary Comparison of Expert and Practitioner Perspectives.

ABSTRACT Assessing and treating a patient's wound is something that every medical student may be faced with from day one of their postgraduate clinical career, regardless of their chosen field of specialisation. This includes acute or post‐surgical wounds as well as chronic wounds. In contrast, learning content concerning wound care is currently underrepresented in medical school curricula, although there are many possible topics and options to incorporate this subject into medical teaching. As...

Wound Care
Erfurt-Berge C, Mahler L, Dissemond J, Krochmann A, Conrad PV•

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